P- and E-cadherin are in separate complexes in cells expressing both cadherins

Exp Cell Res. 1993 Aug;207(2):252-60. doi: 10.1006/excr.1993.1191.


E- and P-cadherin are members of a family of calcium-dependent, cell surface glycoproteins involved in cell-cell adhesion. Extracellularly, the transmembrane cadherins self-associate, while intracellularly, they interact with the actin-based cytoskeleton. Several intracellular proteins, collectively termed catenins, are tightly associated with E- and P-cadherin. These proteins appear to link the cadherin to the cytoskeleton and have been proposed to be involved in concentrating cadherins at cell-cell adherens junctions. In this paper we report the production of monoclonal antibodies against both alpha- and beta-catenin and use these antibodies to show that in cells simultaneously expressing two different cadherins, E-cadherin and P-cadherin, each cadherin appears to be present in a separate cadherin/catenin complex.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Cadherins / analysis*
  • Cadherins / immunology
  • Cadherins / metabolism
  • Carcinoma, Squamous Cell / chemistry
  • Carcinoma, Squamous Cell / metabolism
  • Carcinoma, Squamous Cell / pathology
  • Cell Adhesion / physiology
  • Cell Line
  • Cell Membrane / chemistry
  • Choriocarcinoma / chemistry
  • Choriocarcinoma / metabolism
  • Choriocarcinoma / pathology
  • Cytoskeletal Proteins / metabolism
  • Humans
  • Macrophages / chemistry
  • Macrophages / metabolism
  • Macrophages / pathology
  • Microscopy, Electron
  • Microscopy, Immunoelectron
  • Precipitin Tests
  • Tumor Cells, Cultured
  • alpha Catenin


  • Antibodies, Monoclonal
  • CTNNA1 protein, human
  • Cadherins
  • Cytoskeletal Proteins
  • alpha Catenin