Cloning and characterization of Neisseria meningitidis genes encoding the transferrin-binding proteins Tbp1 and Tbp2

Gene. 1993 Aug 16;130(1):73-80. doi: 10.1016/0378-1119(93)90348-7.


Genes tbp1 and tbp2, encoding the transferrin-binding proteins Tbp1 and Tbp2, have been isolated from two strains of Neisseria meningitidis. The tbp2 and tbp1 open reading frames are tandemly arranged in the genome with an 87-bp intergenic region, and the DNA region upstream from the tbp2-coding sequence contains domains homologous to Escherichia coli promoter consensus motives. Nucleotide sequence analysis suggests the existence of a Tbp1 precursor carrying an N-terminal signal peptide with a peptidase I cleavage site and of a Tbp2 precursor with N-terminal homology to lipoproteins, including a peptidase II cleavage site. Comparison of the Tbp1 deduced amino acid (aa) sequences from both strains showed about 76% aa homology, while those of Tbp2 revealed only about 47% aa homology. These comparisons should be extended to other Neisseria strains in order to evaluate further this genetic divergence further.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, Bacterial / genetics
  • Base Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Carrier Proteins / immunology
  • Cloning, Molecular
  • Conserved Sequence
  • Genes, Bacterial*
  • Gram-Negative Bacteria / genetics
  • Iron-Binding Proteins
  • Lipoproteins / genetics
  • Molecular Sequence Data
  • Neisseria meningitidis / genetics*
  • Open Reading Frames
  • Protein Sorting Signals / genetics
  • Receptors, Transferrin / genetics*
  • Restriction Mapping
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Transferrin / metabolism
  • Transferrin-Binding Proteins


  • Antigens, Bacterial
  • Carrier Proteins
  • Iron-Binding Proteins
  • Lipoproteins
  • Protein Sorting Signals
  • Receptors, Transferrin
  • Transferrin
  • Transferrin-Binding Proteins

Associated data

  • GENBANK/L03653
  • GENBANK/L03654
  • GENBANK/L07632
  • GENBANK/M96932
  • GENBANK/M96933
  • GENBANK/S65693
  • GENBANK/S65694
  • GENBANK/X54209
  • GENBANK/Z15129
  • GENBANK/Z15130
  • GENBANK/Z35133