Sequence of a staphylococcal gene, vat, encoding an acetyltransferase inactivating the A-type compounds of virginiamycin-like antibiotics

Gene. 1993 Aug 16;130(1):91-8. doi: 10.1016/0378-1119(93)90350-c.


The Staphylococcus aureus plasmids, pIP680 and pIP1156, which confer resistance to A-type compounds of virginiamycin-like antibiotics (Vml: streptogramin A, pristinamycin IIA, virginiamycin M) and to synergistic mixtures of the A and B compounds of Vml antibiotics, were shown to direct the modification of A-type compounds by acetylation. The vat gene, encoding the acetyltransferase modifying A-type compounds, was isolated from plasmid pIP680 and sequenced. This gene potentially encodes a 219-amino-acid (aa) protein, VAT, of 24 330 Da showing at least 38% aa identity with two chloramphenicol acetyltransferases encoded by cat genes isolated from Escherichia coli and Agrobacterium tumefaciens. Resistance to A-type compounds of Vml antibiotics conferred to S. aureus by vat was not expressed in E. coli, although a protein having a M(r) similar to that encoded by this gene was detected in E. coli minicells. The vat gene was detected by the polymerase chain reaction in two chromosomally located staphylococcal conjugative elements and in the conjugative plasmid, pIP1156, conferring resistance to A-type compounds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / chemistry
  • Acetyltransferases / genetics*
  • Amino Acid Sequence
  • Bacterial Proteins*
  • Base Sequence
  • Chloramphenicol O-Acetyltransferase / genetics
  • Cloning, Molecular
  • Contraindications
  • DNA, Bacterial / analysis
  • Drug Resistance, Microbial / genetics*
  • Genes, Bacterial*
  • Molecular Sequence Data
  • R Factors / genetics*
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Staphylococcus aureus / enzymology
  • Staphylococcus aureus / genetics*
  • Virginiamycin / pharmacology*


  • Bacterial Proteins
  • DNA, Bacterial
  • Virginiamycin
  • Acetyltransferases
  • Vat protein, Bacteria
  • Chloramphenicol O-Acetyltransferase

Associated data

  • GENBANK/L03653
  • GENBANK/L03654
  • GENBANK/L07632
  • GENBANK/L07778
  • GENBANK/M96932
  • GENBANK/M96933
  • GENBANK/S65693
  • GENBANK/S65694
  • GENBANK/X54209
  • GENBANK/Z35133