Detection of an oestrogen receptor-like protein in human meningiomas by band shift assay using a synthetic oestrogen responsive element (ERE)

Br J Cancer. 1993 Aug;68(2):290-4. doi: 10.1038/bjc.1993.330.

Abstract

When a ligand binding or enzyme immunoassay is used, meningiomas are found to be rich in progestin receptors (PR) whereas oestrogen receptors (ER) are virtually undetectable. A protein that can bind to an oestrogen responsive element (ERE) was detected in meningiomas, by using the band-shift assay. The binding of ER to the ERE is inhibited by the anti-ER monoclonal antibody ER-P31, which is directed to the A/B domain of the ER, indicating that the binding protein is an ER-like protein.

MeSH terms

  • Antibodies, Monoclonal
  • Base Sequence
  • Binding Sites
  • Breast Neoplasms
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Meningeal Neoplasms / metabolism*
  • Meninges / metabolism
  • Meningioma / metabolism*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / metabolism
  • Receptors, Estrogen / analysis*
  • Receptors, Estrogen / metabolism
  • Reference Values
  • Tumor Cells, Cultured

Substances

  • Antibodies, Monoclonal
  • Oligodeoxyribonucleotides
  • Receptors, Estrogen