Properties of alpha 1-antitrypsin secreted by human adenocarcinoma cell lines

FEBS Lett. 1993 Aug 16;328(3):291-5. doi: 10.1016/0014-5793(93)80946-r.


alpha 1-Antitrypsin; (alpha 1-AT) produced by various human carcinoma (non-hepatoma) cell lines were analyzed. Five out of eight cell lines secreted detectable amounts of alpha 1-AT into the conditioned media. All were adenocarcinoma cell lines. The tumor cell-derived alpha 1-ATs had higher molecular weights (MW) than the normal plasma form. Most of this difference was an overall reflection of altered N-glycosylation. As judged by binding of lectins, the glycosylation had shifted towards higher levels of triantennary oligosaccharides and higher levels of fucosylation. The conditioned media also contained lower MW alpha 1-AT species, possibly, proteolytically cleaved forms.

MeSH terms

  • Adenocarcinoma / metabolism*
  • Blotting, Western
  • Chromatography, Affinity
  • Electrophoresis, Polyacrylamide Gel
  • Glycoside Hydrolases
  • Humans
  • Lectins / metabolism
  • Tumor Cells, Cultured
  • alpha 1-Antitrypsin / metabolism*


  • Lectins
  • alpha 1-Antitrypsin
  • Glycoside Hydrolases