The feasibility of examining electro-mechanical activity of the human masseter muscles using non-invasive recording techniques was examined in six healthy dentate adults (aged 34-57 years). Electrical activity of the muscle was examined by surface electromyography (EMG) and the mechanical activity, in the form of muscle sounds, was examined by acoustic myography (AMG). Bilateral recordings of EMG and AMG were made simultaneously using composite probes which were placed on the skin over the masseter muscles. A standardized pressure was applied to the probes via adjustable rods attached to a safety helmet. Pressures were monitored by strain gauges placed between the ends of the rods and the probes. With the subject seated, recordings of AMG and EMG were obtained during maximal jaw clenching for 4 s and the raw signals were stored on a computer. Of three maximal contractions performed, the last two were used in the analysis. The raw amplified signals underwent frequency analysis by fast Fourier Transform. Total activity was also assessed after amplification, full-wave rectification and integration, and repeatability of the results was assessed. The AMG frequency range was 6-15 Hz and was similar to values for other human skeletal muscles. The integrated values for EMG and AMG were repeatable on both sides of the face (IEMG, right r = 0.99, left r = 0.99; IAMG right r = 0.70, left r = 0.71). Simultaneous recordings of AMG and EMG from the masseter muscles may be useful for assessing electro-mechanical muscle function but further validation studies are required before the technique can be used clinically.