Positive selection of T lymphocytes on fibroblasts

Nature. 1993 Aug 12;364(6438):642-5. doi: 10.1038/364642a0.

Abstract

Thymocytes are selected for expression of alpha beta T-cell antigen receptors (TCR) which recognize antigen in conjunction with self-major histocompatibility complex (MHC) molecules. In the thymus the restriction element is imprinted on radioresistant stromal elements and on cells of haematopoietic origin. In mice negative for beta 2-microglobulin that are devoid of mature cytotoxic T lymphocytes, we find that intrathymic injection of different fibroblasts causes the maturation of CD4-CD8+TCRhigh thymocytes with distinct patterns of TCR V beta distribution. Here we show that in TCR-transgenic mice, intrathymic injection of L cells expressing the selecting H-2Kb molecule (L-Kb cells) reconstitutes the maturation of thymocytes bearing the transgenic TCR, and that in normal B10.BR (H-2k) mice, H-2Kb molecules expressed on L-Kb cells lead to the development of T lymphocytes with recognition restricted to H-2Kb. A class I MHC restriction element can thus be selected by interaction with fibroblasts, that is, cells of other than epithelial or haematopoietic origin.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Communication / immunology*
  • Cells, Cultured
  • Fibroblasts / physiology*
  • H-2 Antigens / physiology*
  • Immunophenotyping
  • L Cells
  • Mice
  • Mice, Transgenic
  • Receptors, Antigen, T-Cell, alpha-beta / physiology*
  • T-Lymphocytes / immunology
  • T-Lymphocytes / physiology*
  • beta 2-Microglobulin / physiology

Substances

  • H-2 Antigens
  • Receptors, Antigen, T-Cell, alpha-beta
  • beta 2-Microglobulin