Sequence and functional characterization of feline interleukin 2

Biochem Biophys Res Commun. 1993 Aug 16;194(3):1038-43. doi: 10.1006/bbrc.1993.1926.

Abstract

Since the discovery of its involvement in the pathogenesis of feline immunodeficiency virus infection ("cat AIDS") and feline leukemia virus infection, the role of feline interleukin 2 (IL-2) has been a focus of particular interest. The purpose of this study was to clone feline IL-2 cDNA, as well as synthesize bioactive recombinant feline IL-2. The isolation of cDNA encoding feline IL-2 was carried out using a PCR-based strategy and screening of a feline leukocyte cDNA library. Feline IL-2 consists of 154 amino acids including a putative signal sequence and has 81%, 69%, 60% and 64% identity to human, bovine, murine and rat IL-2, respectively. Feline IL-2 cDNA was expressed in COS-7 cells. The secreted protein has CTLL-4 murine cytotoxic T cell proliferative activity characteristic of authentic IL-2. These data confirm the synthesis of bioactive recombinant feline IL-2.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biological Assay
  • Blotting, Northern
  • Cats
  • Cloning, Molecular
  • Interleukin-2 / biosynthesis*
  • Interleukin-2 / genetics*
  • Interleukin-2 / immunology
  • Leukocytes / immunology
  • Leukocytes / metabolism*
  • Molecular Sequence Data
  • Recombinant Proteins / biosynthesis
  • Sequence Homology, Amino Acid
  • T-Lymphocytes, Cytotoxic / immunology

Substances

  • Interleukin-2
  • Recombinant Proteins

Associated data

  • GENBANK/L19402
  • GENBANK/S63834
  • GENBANK/S63835
  • GENBANK/S63836
  • GENBANK/S63837
  • GENBANK/S63838
  • GENBANK/S64544
  • GENBANK/U11085
  • GENBANK/U11086
  • GENBANK/U11087