cDNA nucleotide sequence and primary structure of mouse uterine peptidylarginine deiminase. Detection of a 3'-untranslated nucleotide sequence common to the mRNA of transiently expressed genes and rapid turnover of this enzyme's mRNA in the estrous cycle

Eur J Biochem. 1993 Aug 1;215(3):677-85. doi: 10.1111/j.1432-1033.1993.tb18079.x.

Abstract

Peptidylarginine deiminase is a protein-modulating enzyme which converts the arginine residues in proteins to citrulline residues. This study describes the complete primary structure of mouse peptidylarginine deiminase, which was deduced from nucleotide sequence analysis of cDNA clones plus proteochemical analysis of the purified enzyme. The composite cDNA sequence contained a 5' untranslated region of 7 bases, an open reading frame of 2019 bases that encoded 673 amino acids, a 3' untranslated region of 2662 bases, and part of a poly(A) tail. The N-terminal and C-terminal sequences of the enzyme matched the sequences deduced from nucleotide analysis. Furthermore, we determined that the N-terminal sequence was N alpha-acetyl-Met-Gln-, a sequence which has never previously been reported among N alpha-acetyl-Met proteins. The Arg 352 of the enzyme was converted to a citrulline residue and the potential Asn-linked glycosylation site (Asn542-Glu543-Ser544) had no carbohydrate moiety. Thus, mouse peptidylarginine deiminase consists of 673 amino acids with a molecular mass of 76,260. Mouse peptidylarginine deiminase mRNA has two AU-rich structures in the 3' untranslated region which exhibit a high degree of similarity to those in lymphokine, cytokine and proto-oncogene mRNA species. Since the rat enzyme (previously reported) does not possess these characteristic structures, we compared the levels of enzyme activity and mRNA in the mouse and rat uterus at four defined phases of the estrous cycle. The degradation of peptidylarginine deiminase and its mRNA proceeded significantly faster in the mouse than in the rat. We speculate that the unusual structure of the mouse enzyme and its mRNA be involved in this species-specific rapid degradation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA
  • Estrus / metabolism*
  • Female
  • Hydrolases / genetics*
  • Introns*
  • Mice
  • Molecular Sequence Data
  • Protein-Arginine Deiminase Type 4
  • Protein-Arginine Deiminases
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Uterus / enzymology*

Substances

  • RNA, Messenger
  • DNA
  • Hydrolases
  • Protein-Arginine Deiminase Type 4
  • Protein-Arginine Deiminases
  • peptidylarginine deiminase 4, mouse

Associated data

  • GENBANK/D16580
  • GENBANK/L09682
  • GENBANK/L09683
  • GENBANK/X71336
  • GENBANK/X71337
  • GENBANK/X71338
  • GENBANK/X71339
  • GENBANK/X71340
  • GENBANK/X72968
  • GENBANK/X74044