Trehalose-6-phosphate, a new regulator of yeast glycolysis that inhibits hexokinases

FEBS Lett. 1993 Aug 23;329(1-2):51-4. doi: 10.1016/0014-5793(93)80191-v.


Trehalose-6-phosphate (P) competitively inhibited the hexokinases from Saccharomyces cerevisiae. The strongest inhibition was observed upon hexokinase II, with a Ki of 40 microM, while in the case of hexokinase I the Ki was 200 microM. Glucokinase was not inhibited by trehalose-6-P up to 5 mM. This inhibition appears to have physiological significance, since the intracellular levels of trehalose-6-P were about 0.2 mM. Hexokinases from other organisms were also inhibited, while glucokinases were unaffected. The hexokinase from the yeast, Yarrowia lipolytica, was particularly sensitive to the inhibition by trehalose-6-P: when assayed with 2 mM fructose an apparent Ki of 5 microM was calculated. Two S. cerevisiae mutants with abnormal levels of trehalose-6-P exhibited defects in glucose metabolism. It is concluded that trehalose-6-P plays an important role in the regulation of the first steps of yeast glycolysis, mainly through the inhibition of hexokinase II.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding, Competitive
  • Fructose / metabolism
  • Glucokinase / antagonists & inhibitors
  • Glucose / metabolism
  • Glycolysis / drug effects*
  • Hexokinase / antagonists & inhibitors*
  • Hexokinase / genetics
  • Mutation
  • Phosphorylation
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Sugar Phosphates / genetics
  • Sugar Phosphates / pharmacology*
  • Trehalose / analogs & derivatives*
  • Trehalose / genetics
  • Trehalose / pharmacology


  • Sugar Phosphates
  • Fructose
  • trehalose-6-phosphate
  • Trehalose
  • Hexokinase
  • Glucokinase
  • Glucose