Inhibition of adhesion of enteroinvasive pathogens to human intestinal Caco-2 cells by Lactobacillus acidophilus strain LB decreases bacterial invasion

FEMS Microbiol Lett. 1993 Jul 1;110(3):299-305. doi: 10.1111/j.1574-6968.1993.tb06339.x.

Abstract

Salmonella typhimurium and enteropathogenic Escherichia coli (EPEC) were found to adhere to the brush border of differentiated human intestinal epithelial Caco-2 cells in culture, whereas Yersinia pseudotuberculosis and Listeria monocytogenes adhered to the periphery of undifferentiated Caco-2 cells. All these enterovirulent strains invaded the Caco-2 cells. Using a heat-killed human Lactobacillus acidophilus (strain LB) which strongly adheres both to undifferentiated and differentiated Caco-2 cells, we have studied inhibition of cell association with and invasion within Caco-2 cells by enterovirulent bacteria. Living and heat-killed Lactobacillus acidophilus strain LB inhibited both cell association and invasion of Caco-2 cells by enterovirulent bacteria in a concentration-dependent manner. The mechanism of inhibition of both adhesion and invasion appears to be due to steric hindrance of human enterocytic pathogen receptors by whole-cell lactobacilli rather than to a specific blockade of receptors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Adhesion / physiology*
  • Binding, Competitive
  • Cells, Cultured
  • Enterobacteriaceae / pathogenicity
  • Enterobacteriaceae / physiology*
  • Enterobacteriaceae / ultrastructure
  • Epithelial Cells
  • Epithelium / microbiology
  • Epithelium / ultrastructure
  • Escherichia coli / pathogenicity
  • Escherichia coli / physiology
  • Escherichia coli / ultrastructure
  • Humans
  • Intestines / cytology
  • Intestines / microbiology*
  • Intestines / ultrastructure
  • Lactobacillus acidophilus / physiology*
  • Lactobacillus acidophilus / ultrastructure
  • Listeria monocytogenes / physiology*
  • Listeria monocytogenes / ultrastructure
  • Microvilli / microbiology*
  • Microvilli / ultrastructure
  • Salmonella typhimurium / pathogenicity
  • Salmonella typhimurium / physiology
  • Salmonella typhimurium / ultrastructure
  • Yersinia pseudotuberculosis / physiology
  • Yersinia pseudotuberculosis / ultrastructure