Human non-secretory ribonucleases. II. Structural characterization of the N-glycans of the kidney, liver and spleen enzymes by NMR spectroscopy and electrospray mass spectrometry

Glycobiology. 1993 Jun;3(3):249-59. doi: 10.1093/glycob/3.3.249.


The N-glycans have been removed by peptide-N-glycosidase F (PNGase F) from purified human non-secretory RNases derived from kidney, liver and spleen. The spleen RNase was purified by two procedures, one of which did not include the usual acid treatment step (0.25 M H2SO4, 45 min, 4 degrees C), to determine if acid treatment alters the carbohydrate moieties. The N-glycans of the RNases were fractionated by Bio-Gel P-4 chromatography and analysed by 600 MHz 1H-NMR spectroscopy and electrospray mass spectrometry. All four non-secretory RNase preparations contained the following structures: [formula: see text] The relative amounts of the trisaccharide, pentasaccharide and hexasaccharide appeared to vary slightly in the different tissue RNases. The overall results indicate: (i) that acid treatment during purification does not alter the N-glycans of non-secretory RNases; (ii) that the N-glycans from kidney, liver and spleen non-secretory RNases are very similar, if not identical, to one another, but different from the N-glycan structures reported for secretory RNase.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Carbohydrate Sequence
  • Carbohydrates / analysis
  • Electrophoresis, Polyacrylamide Gel
  • Glycoproteins / chemistry*
  • Glycoproteins / isolation & purification
  • Glycoproteins / metabolism
  • Humans
  • Kidney / enzymology*
  • Liver / enzymology*
  • Magnetic Resonance Spectroscopy / methods
  • Mass Spectrometry
  • Molecular Sequence Data
  • Molecular Weight
  • Oligosaccharides / chemistry*
  • Oligosaccharides / isolation & purification
  • Polysaccharides / chemistry*
  • Polysaccharides / isolation & purification
  • Ribonucleases / chemistry*
  • Ribonucleases / isolation & purification
  • Ribonucleases / metabolism
  • Spleen / enzymology*


  • Carbohydrates
  • Glycoproteins
  • Oligosaccharides
  • Polysaccharides
  • Ribonucleases