Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. Jul-Aug 1993;24(1-2):21-5.
doi: 10.1007/BF00324660.

The Glycerol Kinase (GUT1) Gene of Saccharomyces Cerevisiae: Cloning and Characterization

Affiliations

The Glycerol Kinase (GUT1) Gene of Saccharomyces Cerevisiae: Cloning and Characterization

P Pavlik et al. Curr Genet. .

Abstract

The GUT1 gene of Saccharomyces cerevisiae, encoding glycerol kinase, was cloned and sequenced. The cloned genomic DNA fragment contains an open reading frame potentially coding for a protein of 709 amino acids with homology to bacterial glycerol kinases (40.8% identity over 502 amino acids, and 42.1% identity over 496 amino acids, in comparison to the smaller E. coli and B. subtilis enzymes). Disruption of GUT1 showed that the gene is required for growth on glycerol, but not on glucose or ethanol media. No glycerol kinase activity was detected in the disruption mutant. According to enzyme activity and transcript analysis, synthesis of glycerol kinase is repressed by glucose, and derepression is ADR1-dependent.

Similar articles

See all similar articles

Cited by 23 articles

See all "Cited by" articles

References

    1. Eur J Biochem. 1968 Jul;5(2):165-72 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7826-30 - PubMed
    1. Comput Appl Biosci. 1988 Mar;4(1):11-7 - PubMed
    1. Mol Cell Biol. 1989 Jan;9(1):34-42 - PubMed
    1. Mol Cell Biol. 1991 Mar;11(3):1566-77 - PubMed

Publication types

MeSH terms

LinkOut - more resources

Feedback