A transformant of Aspergillus fumigatus deficient in the antigenic cytotoxin ASPFI

FEMS Microbiol Lett. 1993 Jul 15;111(1):31-6. doi: 10.1111/j.1574-6968.1993.tb06357.x.

Abstract

The aspfI gene encoding a ribonucleotoxin, a putative virulence factor of Aspergillus fumigatus, was inactivated by gene disruption. Gene replacement through homologous recombination by the disrupted allele tagged by the hygromycin B resistance marker was performed by transformation of a pathogenic strain. One transformant with the disrupted aspfI gene failed to produce the ASPFI protein and was shown to be pathogenic for mice. We concluded that this ribotoxin is not a main factor in the colonization of the lung tissues by A. fumigatus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens*
  • Animals
  • Antigens, Plant
  • Aspergillosis / microbiology
  • Aspergillus fumigatus / genetics*
  • Aspergillus fumigatus / isolation & purification
  • Aspergillus fumigatus / pathogenicity
  • Chromosomes, Fungal
  • Cloning, Molecular
  • Cytotoxins / genetics*
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • Fungal Proteins / analysis
  • Fungal Proteins / biosynthesis
  • Fungal Proteins / genetics*
  • Genes, Fungal
  • Humans
  • Mice
  • Plasmids
  • Restriction Mapping
  • Ribonucleases*
  • Transformation, Genetic*
  • Virulence / genetics

Substances

  • ASPF1 protein, Aspergillus fumigatus
  • Allergens
  • Antigens, Plant
  • Cytotoxins
  • DNA, Fungal
  • Fungal Proteins
  • MITF protein, Aspergillus restrictus
  • Ribonucleases