Interferon beta decreases T cell activation and interferon gamma production in multiple sclerosis

J Neuroimmunol. 1993 Jul;46(1-2):145-53. doi: 10.1016/0165-5728(93)90244-s.


Interferons (IFN) are biological molecules with anti-viral, anti-proliferative and immunomodulatory actions. There is evidence that IFN-gamma increases the frequency of exacerbations of multiple sclerosis (MS) whereas IFN-beta may reduce their frequency. Here we present evidence that IFN-beta significantly decreases concanavalin A (Con A)-induced proliferation of peripheral blood mononuclear cells (PBMC) of MS patients and healthy individuals. Similar results were obtained when PBMC were activated through the T cell receptor (TcR) by anti-CD3 monoclonal antibody or independently of it by phorbol ester and Ca2+ ionophore. These effects of IFN-beta were also noted when IFN-gamma and IFN-beta were added together. Furthermore, IFN-beta decreased proliferation when added to cells that were already pre-activated. Activated CD4+ and CD8+ T cells were downregulated to approximately the same extent. Analysis of cytokine production showed that IFN-gamma production by Con A activated PBMC was increased in MS when compared to controls. IFN-beta significantly decreased IFN-gamma production in MS patients and control individuals. Con A activated cultures treated with IFN-beta showed decreased IL2R expression and accumulation of IL2. These results show that IFN-beta decreases T cell activation and IFN-gamma production in vitro, effects that may be beneficial in MS.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Down-Regulation
  • Humans
  • Interferon-beta / pharmacology*
  • Interferon-gamma / biosynthesis*
  • Interleukin-2 / physiology
  • Lymphocyte Activation / drug effects*
  • Middle Aged
  • Multiple Sclerosis / immunology*
  • Receptors, Interleukin-2 / metabolism
  • Recombinant Proteins / pharmacology
  • T-Lymphocytes / immunology*


  • Interleukin-2
  • Receptors, Interleukin-2
  • Recombinant Proteins
  • Interferon-beta
  • Interferon-gamma