Trafficking of activated cytotoxic T lymphocytes into the central nervous system: use of a transgenic model

M B Oldstone; P J Southern

doi:10.1016/0165-5728(93)90230-v

Trafficking of activated cytotoxic T lymphocytes into the central nervous system: use of a transgenic model

J Neuroimmunol. 1993 Jul;46(1-2):25-31. doi: 10.1016/0165-5728(93)90230-v.

Authors

M B Oldstone¹, P J Southern

Affiliation

¹ Department of Neuropharmacology, Scripps Research Institute, La Jolla, CA 92037.

Abstract

We have used cell or tissue-specific promoters to express lymphocytic choriomeningitis virus (LCMV) proteins in selected cells in independent lines of transgenic mice. Upon adoptive transfers into these mice, MHC-restricted LCMV-specific cytotoxic T lymphocytes homed specifically to either the choroid plexus (SV40 promoter) or beta cells of the islets of Langerhans (rat insulin promoter). The availability of promoters specific for neurons, oligodendrocytes and astrocytes makes this approach compelling for evaluating T cell trafficking into the CNS and for analyzing antigen presentation in vivo in the CNS.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Amino Acid Sequence
Animals
Antigens, Viral / chemistry*
Antigens, Viral / genetics
Cell Movement
Choroid Plexus / cytology
Choroid Plexus / immunology*
Gene Expression
Immunity, Cellular
Islets of Langerhans / immunology
Lymphocytic choriomeningitis virus / chemistry
Lymphocytic choriomeningitis virus / immunology
Mice
Mice, Transgenic
Molecular Sequence Data
T-Lymphocytes, Cytotoxic / cytology*
Viral Proteins / genetics
Viral Proteins / immunology

Substances

Antigens, Viral
Viral Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding