Cytotoxic effect of hinokitiol and tropolone on the growth of mammalian cells and on blastogenesis of mouse splenic T cells

Biol Pharm Bull. 1993 May;16(5):521-3. doi: 10.1248/bpb.16.521.


Hinokitiol (I) and tropolone (II) showed characteristic cytotoxic effects in vitro on five kinds of human and murine cell lines and blastic lymphocytes from mouse splenocytes. The cytotoxic effect of I on the growth of murine and human tumor cell lines, including RL male-1, MH134, HL60, K562 and KATO-III was definite when examined by thymidine incorporation into DNA and its 50% inhibitory concentration (IC50) on all cells was 0.3-0.6 microgram/ml. Compound II also showed comparable cytotoxic effects on these cell lines, indicating a little lower activity when compared to I. Furthermore, I and II also completely suppressed the [3H]thymidine ([3H]TdR) incorporation of mitogen-induced blastic lymphocytes. The suppressive activity on mouse lymphocyte proliferative response to concanavaline-A was also found with both compounds at a low concentration of 0.32 microgram/ml. As compound I is known to be of fairly low toxicity (LD50: 453 + 24 mg/kg in mice), the antitumor and immuno-suppressive effect of hinokitiol (I) should be further investigated.

MeSH terms

  • Animals
  • Cell Division / drug effects
  • Cell Survival / drug effects
  • Concanavalin A / pharmacology
  • Humans
  • Lymphocyte Activation / drug effects*
  • Male
  • Mice
  • Mice, Inbred C3H
  • Monoterpenes*
  • Spleen / cytology*
  • Spleen / drug effects
  • T-Lymphocytes / drug effects*
  • T-Lymphocytes / metabolism
  • Thymidine / metabolism
  • Tropolone / analogs & derivatives*
  • Tropolone / pharmacology
  • Tumor Cells, Cultured


  • Monoterpenes
  • Concanavalin A
  • Tropolone
  • beta-thujaplicin
  • Thymidine