ADP-ribosylation of nucleolar proteins in HeLa tumor cells

J Cell Biochem. 1993 Jun;52(2):153-8. doi: 10.1002/jcb.240520207.

Abstract

ADP-ribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with 32P-NAD; then the nucleolar proteins were analyzed by 1-dimensional and 2-dimensional polyacrylamide gel electrophoresis (PAGE) and modified proteins were detected by autoradiography. The labeled nucleolar proteins were also chromatographically fractionated on DEAE-cellulose. Electrophoretic analysis of total nucleolar and chromatographically purified proteins revealed that besides nuclear ADP-ribosyltransferase and histones two characteristic nucleolar phosphoproteins numatrin/B23 and nucleolin/C23 were modified by ADP-ribosylation.

MeSH terms

  • Adenosine Diphosphate Ribose / metabolism*
  • Cell Division
  • Electrophoresis, Polyacrylamide Gel
  • HeLa Cells
  • Humans
  • Nuclear Proteins / metabolism*

Substances

  • Nuclear Proteins
  • Adenosine Diphosphate Ribose