Detection of iron binding proteins by a blotting technique

Anal Biochem. 1993 Jul;212(1):47-9. doi: 10.1006/abio.1993.1289.

Abstract

We describe a simple method for detecting iron binding proteins based on binding of 59Fe to protein blots from SDS gels followed by autoradiography. This binding is specific and is not affected by other metal ions. The method has allowed identification of new iron binding proteins in intestinal mucosa and in Hela cells.

MeSH terms

  • Animals
  • Carrier Proteins / chemistry
  • Carrier Proteins / isolation & purification*
  • Collodion
  • Electrophoresis, Polyacrylamide Gel
  • Evaluation Studies as Topic
  • HeLa Cells
  • Humans
  • Intestinal Mucosa / chemistry
  • Iron / metabolism*
  • Iron-Binding Proteins
  • Molecular Weight
  • Protein Binding
  • Rats
  • Tissue Distribution
  • Transferrin-Binding Proteins

Substances

  • Carrier Proteins
  • Iron-Binding Proteins
  • Transferrin-Binding Proteins
  • Collodion
  • Iron