Association between the effacing (eae) gene and the Shiga-like toxin-encoding genes in Escherichia coli isolates from cattle

Am J Vet Res. 1993 Jul;54(7):1064-8.

Abstract

Two hundred ninety-six Escherichia coli isolates from feces or intestines of calves with diarrhea were hybridized with 7 gene probes. One probe (the eae probe) was derived from the eae gene coding for a protein involved in the effacement of the enterocyte microvilli by the group of bacteria called attaching and effacing E coli (AEEC), and 2 probes were derived from genes coding for the Shiga-like toxins (SLT) 1 and 2 produced by the verocytotoxic E coli (VTEC). The other 4 probes were derived from DNA sequences associated with the adhesive properties of enteroadherent E coli (EAEC) to cultured cells (the EAF probe for the localized adherence pattern, probes F1845 and AIDA-1 for the diffuse adherence pattern, and the Agg probe for the aggregative adherence pattern). Hybridization results for the eae probe were in agreement, for all but 1 of the 8 isolates, with previously published phenotypic results of microvilli effacement. The latter was previously reported as effacing the microvilli of calf enterocytes, but was eae probe-negative. Two classes of isolates hybridized with the eae probe. Members of a first class (60 isolates) additionally produced a positive signal with 1 or both of the SLT probes (VTEC-AEEC isolates). Isolates hybridizing with the eae and the SLT1 probes were the most frequent: 56 isolates (ie, 93% of all VTEC-AEEC). Members of the second class (10 isolates) failed to hybridize with either SLT probe (non-VTEC-AEEC isolates).(ABSTRACT TRUNCATED AT 250 WORDS)

MeSH terms

  • Animals
  • Bacterial Toxins / genetics*
  • Cattle / microbiology*
  • DNA Probes
  • DNA, Neoplasm / genetics
  • Enterotoxins / genetics*
  • Escherichia coli / classification
  • Escherichia coli / genetics*
  • Escherichia coli / isolation & purification
  • Genes, Bacterial*
  • Plasmids
  • Restriction Mapping
  • Serotyping
  • Shiga Toxin 1
  • Shiga Toxin 2

Substances

  • Bacterial Toxins
  • DNA Probes
  • DNA, Neoplasm
  • Enterotoxins
  • Shiga Toxin 1
  • Shiga Toxin 2