Characterization of a new class of tetracycline-resistance gene tet(S) in Listeria monocytogenes BM4210

Gene. 1993 Sep 6;131(1):27-34. doi: 10.1016/0378-1119(93)90665-p.

Abstract

The nucleotide sequence of the tetracycline (Tc)-minocycline (Mc)-resistance determinant of plasmid pIP811 from Listeria monocytogenes BM4210 has been determined. The gene, designated tet(S), was identified by analysis of the start and stop codons as a coding sequence of 1923 bp, corresponding to a protein with a calculated M(r) of 72,912. The apparent 68-kDa size estimated by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis of the protein characterized in a cell-free coupled transcription-translation system was in good agreement with the calculated value. The tet(S) gene product exhibits 79 and 72% amino acid identity with Tet(M) from Streptococcus pneumoniae and Tet(O) from Campylobacter coli, respectively. The distribution of tet(S) in strains of Gram+ and Gram- genera resistant to Tc (TcR) and Mc (McR) was studied by hybridization under high stringency using a 590-bp intragenic probe. Homology with tet(S) was detected in two clinical isolates of L. monocytogenes isolated in different geographical areas.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Campylobacter coli / drug effects
  • Campylobacter coli / genetics
  • Carrier Proteins*
  • Cloning, Molecular
  • DNA, Bacterial / analysis
  • Genes, Bacterial*
  • Gram-Negative Bacteria / drug effects
  • Gram-Negative Bacteria / genetics
  • Gram-Positive Bacteria / drug effects
  • Gram-Positive Bacteria / genetics
  • Listeria monocytogenes / drug effects*
  • Listeria monocytogenes / genetics*
  • Membrane Transport Proteins*
  • Minocycline / pharmacology
  • Molecular Sequence Data
  • Plasmids
  • Protein Biosynthesis
  • R Factors*
  • RNA, Ribosomal, 16S
  • Restriction Mapping
  • Ribosomes / drug effects
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Streptococcus pneumoniae / drug effects
  • Streptococcus pneumoniae / genetics
  • Tetracycline / pharmacology
  • Tetracycline Resistance / genetics*
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • Carrier Proteins
  • DNA, Bacterial
  • Membrane Transport Proteins
  • RNA, Ribosomal, 16S
  • Tet M resistance protein, Bacteria
  • Tet O resistance protein, Bacteria
  • Tet(S) protein, Listeria monocytogenes
  • Tetracycline
  • Minocycline

Associated data

  • GENBANK/L09756
  • GENBANK/L11622
  • GENBANK/L28752
  • GENBANK/X71369
  • GENBANK/X71370
  • GENBANK/X71371
  • GENBANK/X71372
  • GENBANK/X71373
  • GENBANK/X71374
  • GENBANK/X71375