We have recorded 404 single units extracellularly in the cerebellar cortex of the rat with tungsten microelectrodes. Waveforms of action potentials were analyzed in order to develop criteria for on-line identification of cell types. Two of the four most frequently recorded waveforms were simple and complex spikes from Purkinje cells. The other two originated from granule cells and glomeruli. Presumed granule cells showed biphasic action potentials with half-widths (0.78 +/- 0.14 ms, n = 51) broader than those of the simple spikes of Purkinje cells (0.22 +/- 0.06 ms, n = 54), whereas presumed glomerular potentials had complex action potentials with narrower half-widths (0.14 +/- 0.05 ms, n = 35). The mean inter-spike interval of presumed granule cells (333.3 +/- 195.4 ms, n = 53) was longer than that of Purkinje cells (47.3 +/- 31.8 ms, n = 59) and the presumed glomerular potentials (77.7 +/- 50.8 ms, n = 20). Results were virtually identical from 17 cerebellar units recorded extracellularly in the cat. Intracellular recording and staining of 20 granule cells with HRP-filled microelectrodes provided further support for our assessment. These results suggest that action potentials from granule cells may be identified on-line by waveform.