Homologs of the synaptobrevin/VAMP family of synaptic vesicle proteins function on the late secretory pathway in S. cerevisiae

Cell. 1993 Sep 10;74(5):855-61. doi: 10.1016/0092-8674(93)90465-3.

Abstract

The yeast S. cerevisiae possesses two genes, SNC1 and SNC2, that encode homologs of vertebrate synaptic vesicle-associated membrane proteins, also known as synaptobrevins. Here we describe the cloning of SNC2 and demonstrate that yeast lacking both SNC genes are deficient in normal bulk secretion, accumulate large numbers of post-Golgi vesicles, and display a variety of conditional lethal phenotypes. In addition, we show that yeast Snc proteins localize to post-Golgi transport vesicles that accumulate in a late-acting sec mutant. Our findings clearly place the Snc proteins on the late secretory pathway in S. cerevisiae and provide direct in vivo evidence that synaptobrevin-like proteins mediate the targeting and transport of secretory proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA, Fungal / genetics
  • DNA, Fungal / metabolism
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Gene Deletion
  • Genes, Fungal*
  • Glycoside Hydrolases / biosynthesis
  • Golgi Apparatus / metabolism
  • Golgi Apparatus / ultrastructure
  • Humans
  • Kinetics
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Microscopy, Immunoelectron
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / metabolism
  • Oligodeoxyribonucleotides
  • R-SNARE Proteins
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae / ultrastructure
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Vertebrates
  • beta-Fructofuranosidase

Substances

  • DNA, Fungal
  • Fungal Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Oligodeoxyribonucleotides
  • R-SNARE Proteins
  • SNC1 protein, S cerevisiae
  • SNC2 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Glycoside Hydrolases
  • beta-Fructofuranosidase

Associated data

  • GENBANK/L16243
  • GENBANK/L20946
  • GENBANK/L20947
  • GENBANK/L22475
  • GENBANK/L33709
  • GENBANK/S60902
  • GENBANK/S60903
  • GENBANK/S60904
  • GENBANK/S60905
  • GENBANK/S60924