Crystallization and preliminary X-ray analysis of a microbial superantigen staphylococcal enterotoxin C2

E F Passalacqua; R D Brehm; K R Acharya; H S Tranter

doi:10.1006/jmbi.1993.1493

Crystallization and preliminary X-ray analysis of a microbial superantigen staphylococcal enterotoxin C2

J Mol Biol. 1993 Sep 5;233(1):170-2. doi: 10.1006/jmbi.1993.1493.

Authors

E F Passalacqua¹, R D Brehm, K R Acharya, H S Tranter

Affiliation

¹ Department of Biochemistry, University of Bath, Claverton Down, Avon, U.K.

PMID: 8377185
DOI: 10.1006/jmbi.1993.1493

Abstract

High yields of staphylococcal enterotoxin C2, from Staphylococcus aureus, have been purified using dye ligand chromatography. Crystals suitable for X-ray diffraction work were obtained by vapour diffusion using ammonium sulphate and polyethylene glycol as precipitants. They belong to the tetragonal space group P4(1)22 with unit cell dimensions a = b = 43.2 A and c = 290.9 A with one molecule per asymmetric unit. The crystals diffract to at least 2.8 A resolution and are suitable for three-dimensional X-ray structural analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens, Bacterial / chemistry*
Antigens, Bacterial / isolation & purification
Enterotoxins / chemistry*
Enterotoxins / isolation & purification
X-Ray Diffraction

Substances

Antigens, Bacterial
Enterotoxins
enterotoxin C, staphylococcal