Yeast GAL11 protein is a distinctive type transcription factor that enhances basal transcription in vitro

Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8382-6. doi: 10.1073/pnas.90.18.8382.

Abstract

The yeast auxiliary transcription factor GAL11, a candidate for the coactivator, was partially purified from yeast cells, and its function was characterized in a cell-free transcription system. The partially purified GAL11 protein stimulated basal transcription from the CYC1 core promoter by a factor of 4-5 at the step of preinitiation complex formation. GAL11 protein also enhanced transcription activated by general regulatory factor 1, GAL4-AH, or GAL4-VP16 to the same extent as the basal transcription. Therefore, the apparent potentiation of the activators by GAL11 was attributable to the stimulation of basal transcription. The wild-type GAL11 protein (but not a mutant-type protein) produced in bacteria stimulated transcription as effectively as GAL11 from yeast. These results suggest that GAL11 functions as a positive cofactor of basal and activator-induced transcription in a cell-free transcription system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / metabolism
  • DNA, Fungal / metabolism
  • Fungal Proteins / biosynthesis
  • Fungal Proteins / isolation & purification
  • Fungal Proteins / metabolism*
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Immunoblotting
  • Kinetics
  • Mediator Complex
  • Mutagenesis
  • Plasmids
  • Promoter Regions, Genetic
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • TATA Box
  • Trans-Activators*
  • Transcription Factors / biosynthesis
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism*
  • Transcription, Genetic*

Substances

  • DNA, Fungal
  • Fungal Proteins
  • GAL11 protein, S cerevisiae
  • Mediator Complex
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factors