A single-strand-specific DNA-binding protein from mouse cells that stimulates DNA polymerase

Eur J Biochem. 1977 Feb 15;73(1):17-24. doi: 10.1111/j.1432-1033.1977.tb11287.x.


A protein of 30000-35000 molecular weight was isolated from mouse ascites cells. This protein binds preferentially to single-stranded DNA. Evidence is presented that this protein maintains single-stranded DNA in an extended configuration. In the presence of single-stranded template the protein stimulates mammalian DNA polymerase alpha but not the mammalian DNA polymerase beta and not some microbial DNA polymerases. The protein is phosphorylated in vitro by a chromatin-associated protein kinase. The modified DNA-binding protein does not stimulate the DNA polymerase alpha.

MeSH terms

  • Animals
  • Carcinoma, Ehrlich Tumor / enzymology
  • Carrier Proteins / isolation & purification
  • Carrier Proteins / physiology*
  • DNA, Single-Stranded / metabolism*
  • DNA, Viral / metabolism
  • DNA-Directed DNA Polymerase / metabolism*
  • Enzyme Activation
  • Kinetics
  • Mice
  • Phosphoproteins / metabolism*
  • Sodium Chloride


  • Carrier Proteins
  • DNA, Single-Stranded
  • DNA, Viral
  • Phosphoproteins
  • Sodium Chloride
  • DNA-Directed DNA Polymerase