A radioimmunoassay for human urinary prealbumin

Ann Clin Biochem. 1993 Jul:30 ( Pt 4):377-82. doi: 10.1177/000456329303000405.

Abstract

We describe a validated radioimmunoassay for prealbumin in urine. Using timed overnight urine samples, the normal reference range was less than 10-148 micrograms/L; or less than 1.8-9.6 micrograms/mmol creatinine, excretion in women being significantly greater than in men (P < 0.05); or less than 7.3-114 ng/min with no significant difference in excretion rate between the sexes. Urines exhibited loss of immunoreactivity after storage at -20 degrees C and thawing. No such loss occurred after storage for 4 weeks at 4 degrees C or room temperature. The urinary excretion of prealbumin was highly correlated with that of albumin (r = 0.85), and clearance relative to creatinine was 2 x 10(-6), the same order as that of albumin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Creatinine / urine
  • Cross Reactions
  • Female
  • Humans
  • Male
  • Middle Aged
  • Prealbumin / urine*
  • Radioimmunoassay*
  • Reference Values

Substances

  • Prealbumin
  • Creatinine