cPLA2 is phosphorylated and activated by MAP kinase

Cell. 1993 Jan 29;72(2):269-78. doi: 10.1016/0092-8674(93)90666-e.


Treatment of cells with agents that stimulate the release of arachidonic acid causes increased serine phosphorylation and activation of cytosolic phospholipase A2 (cPLA2). Here we report that cPLA2 is a substrate for mitogen-activated protein (MAP) kinase. Moreover, phosphorylation by MAP kinase increases the enzymatic activity of cPLA2. The site of cPLA2 phosphorylation by MAP kinase, Ser-505, is identical to the major site of cPLA2 phosphorylation observed in phorbol ester-treated cells. Replacement of Ser-505 with Ala resulted in a mutant cPLA2 that is not a substrate for MAP kinase and causes little or no enhanced agonist-stimulated arachidonate release from intact cells. Taken together, these data indicate that MAP kinase mediates, at least in part, the agonist-induced activation of cPLA2.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CHO Cells
  • Calcimycin / pharmacology
  • Calcium / metabolism
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Cell Line
  • Cricetinae
  • Cytosol / enzymology
  • Enzyme Activation
  • Kinetics
  • Models, Biological
  • Mutagenesis, Site-Directed
  • Peptide Mapping
  • Phospholipases A / genetics
  • Phospholipases A / isolation & purification
  • Phospholipases A / metabolism*
  • Phospholipases A2
  • Phosphopeptides / isolation & purification
  • Phosphorylation
  • Protein Kinase C / metabolism
  • Protein Kinases / metabolism*
  • Receptors, Platelet-Derived Growth Factor / physiology
  • Recombinant Proteins / metabolism
  • Serine
  • Tetradecanoylphorbol Acetate / pharmacology
  • Transfection


  • Phosphopeptides
  • Recombinant Proteins
  • Calcimycin
  • Serine
  • Protein Kinases
  • Receptors, Platelet-Derived Growth Factor
  • Protein Kinase C
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Phospholipases A
  • Phospholipases A2
  • Tetradecanoylphorbol Acetate
  • Calcium