Lung mast cells increase in number and degranulate during pulmonary artery occlusion/reperfusion injury in dogs

Am Rev Respir Dis. 1993 Feb;147(2):448-56. doi: 10.1164/ajrccm/147.2.448.

Abstract

The role of mast cells in pulmonary artery occlusion/reperfusion injury was examined. Lung tissue was obtained from dogs after left pulmonary artery occlusion for 48 h (n = 5) or after similar occlusion followed by 4 h of reperfusion (n = 11). By light microscopy and morphometry, the percentage of mast cells increased 2.4-fold (p < 0.05) in nonoccluded right lungs and 2.9-fold (p < 0.05) in occluded left lungs without reperfusion compared with that in control lungs. After reperfusion, the occluded left lung contained 1.8-fold (p < 0.05) as many mast cells as the nonoccluded right lung and 4.2-fold (p < 0.05) more than that in control lungs. Hydroxyurea did not significantly affect the number of mast cells observed in the right and left lungs after ischemia/reperfusion; 39.8% and 54.4% of the mast cells were degranulated in nonoccluded right lung and occluded left lung preparations, respectively, after left pulmonary artery ischemia/reperfusion (each, p < 0.05 compared with control lungs). The release of eicosanoids into the airways during ischemia/reperfusion injury was also examined. Thromboxane B2 and leukotriene B4 were markedly increased (each, p < 0.05 compared with that in control lungs) in bronchial lavage fluids from both nonoccluded and occluded lungs compared with sham-occluded lungs. Thus, mast cell recruitment and degranulation may play a role in lung ischemia/reperfusion injury.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arterial Occlusive Diseases / metabolism
  • Arterial Occlusive Diseases / pathology*
  • Bronchoalveolar Lavage Fluid / chemistry
  • Cell Count / drug effects
  • Cell Degranulation* / drug effects
  • Dogs
  • Hydroxyurea / pharmacology
  • Leukotriene B4 / analysis
  • Lung / drug effects
  • Lung / pathology*
  • Mast Cells / drug effects
  • Mast Cells / pathology*
  • Microscopy, Electron
  • Pulmonary Artery*
  • Reperfusion Injury / metabolism
  • Reperfusion Injury / pathology*
  • SRS-A / analysis
  • Thromboxane B2 / analysis
  • Time Factors

Substances

  • SRS-A
  • Leukotriene B4
  • Thromboxane B2
  • Hydroxyurea