Distribution of beta 2-adrenergic receptor mRNA expression along the hamster nephron segments

FEBS Lett. 1993 Feb 22;318(1):65-70. doi: 10.1016/0014-5793(93)81329-x.

Abstract

Distribution of beta 2-adrenergic receptor mRNA expression along the microdissected hamster nephron segments was examined by the reverse transcription-polymerase chain reaction (RT-PCR) technique. Conventional RT-PCR using a set of primers on separate exons could not be applied for the detection of beta 2-adrenergic receptor mRNA because of its intronless nature. We used the 'rapid amplification of cDNA ends' protocol [(1985) Proc. Natl. Acad. Sci. USA 85, 8998-9002] as a maneuver for RT-PCR of an intronless gene. Using this method, we successfully located hamster beta 2-adrenergic receptor mRNA only in glomeruli and early proximal convoluted tubule along the nephron segments tested.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cricetinae
  • DNA
  • Female
  • Introns
  • Male
  • Mesocricetus
  • Molecular Sequence Data
  • Nephrons / metabolism*
  • Polymerase Chain Reaction
  • RNA, Messenger / metabolism*
  • Receptors, Adrenergic, beta / genetics*

Substances

  • RNA, Messenger
  • Receptors, Adrenergic, beta
  • DNA