Isolation of a cDNA specifying subunit VIIb of human cytochrome c oxidase

Biochim Biophys Acta. 1993 Feb 20;1172(1-2):223-5. doi: 10.1016/0167-4781(93)90301-s.


Human cytochrome c oxidase (COX) is a complex of 13 subunits: three are encoded by mitochondrial DNA and ten by nuclear DNA. We have now isolated a full-length cDNA specifying subunit VIIb, the last remaining uncharacterized nuclear-encoded subunit cDNA of human COX. The cDNA encodes a deduced 80-aa polypeptide, including a 24-amino acid (aa) N-terminal leader sequence and a 56-aa mature polypeptide with 82% identity to mature bovine COX VIIb. Southern blot hybridization of human muscle genomic DNA showed multiple hybridizing bands, implying the presence of a large coxVIIb gene family, including a potential processed pseudogene.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Mitochondrial / genetics*
  • DNA, Mitochondrial / isolation & purification
  • Electron Transport Complex IV / genetics*
  • Humans
  • Macromolecular Substances
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid


  • DNA, Mitochondrial
  • Macromolecular Substances
  • RNA, Messenger
  • Electron Transport Complex IV

Associated data

  • GENBANK/D13131
  • GENBANK/D13132
  • GENBANK/D13133
  • GENBANK/D13134
  • GENBANK/D13135
  • GENBANK/D13136
  • GENBANK/D13137
  • GENBANK/U08372
  • GENBANK/U09362
  • GENBANK/Z14244