Conditional dihydrofolate reductase deficiency due to transposon Tn5tac1 insertion downstream from the folA gene in Escherichia coli

Gene. 1993 Mar 15;125(1):69-73. doi: 10.1016/0378-1119(93)90747-q.

Abstract

Transposon Tn5tac1 can generate conditional mutations by virtue of an outward-facing tac promoter, which is regulated by the lac repressor and isopropyl-beta-D-thiogalactopyranoside (IPTG). We report here on a Tn5tac1 insertion in Escherichia coli that results in a conditional (IPTG-elicited) folA mutant phenotype: During aerobic growth, IPTG caused decreased synthesis of dihydrofolate reductase (DHFR; encoded by the folA gene) and hypersensitivity to trimethoprim (a DHFR inhibitor); during anaerobic growth, IPTG elicited auxotrophy that was satisfied by thymine or glycine or threonine. The Tn5tac1 insertion was downstream from folA, with the tac promoter pointing into the gene (antisense direction). Complementation tests indicated that the conditional folA deficiency was a cis effect of transcription from the tac promoter, perhaps due to head-to-head collision between converging RNA polymerases.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Base Sequence
  • DNA Transposable Elements*
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Isopropyl Thiogalactoside
  • Molecular Sequence Data
  • Mutation
  • Restriction Mapping
  • Tetrahydrofolate Dehydrogenase / deficiency*
  • Tetrahydrofolate Dehydrogenase / genetics

Substances

  • DNA Transposable Elements
  • Isopropyl Thiogalactoside
  • Tetrahydrofolate Dehydrogenase