Growth factor function of vasoactive intestinal peptide in whole cultured mouse embryos

Nature. 1993 Mar 11;362(6416):155-8. doi: 10.1038/362155a0.


Factors controlling central nervous system (CNS) growth immediately after neurulation are mostly unknown. Vasoactive intestinal peptide (VIP) receptors are widely distributed in the embryonic nervous system, and VIP has trophic and mitogenic properties on embryonic neural tissues but inhibits growth and mitosis in certain tumours. To address the potential effects of VIP on embryonic growth, we used whole postimplantation embryo cultures. After a 4-h incubation, VIP stimulated growth, increasing somite number, embryonic volume, DNA and protein content, and number of cells in S-phase. A VIP antagonist substantially inhibited these VIP-mediated increments in growth. The VIP antagonist completely suppressed VIP-stimulated mitosis in the CNS while decreasing the same in non-neuronal tissues by 38%. In vitro autoradiography revealed GTP-sensitive and GTP-insensitive VIP receptors which were differentially regulated in VIP antagonist-treated embryos. The present study suggests that VIP acts as a growth factor on early postimplantation embryos through multiple VIP receptors that exhibit tissue-specific responses.

MeSH terms

  • Analysis of Variance
  • Animals
  • Cell Division / drug effects
  • Central Nervous System / drug effects
  • Central Nervous System / embryology
  • DNA / metabolism
  • Embryo, Mammalian / drug effects*
  • Embryo, Mammalian / physiology
  • GTP-Binding Proteins / physiology
  • Growth Substances / pharmacology*
  • Mice
  • Organ Culture Techniques
  • Proteins / metabolism
  • Receptors, Gastrointestinal Hormone / metabolism
  • Receptors, Vasoactive Intestinal Peptide
  • S Phase / drug effects
  • Secretin / pharmacology
  • Vasoactive Intestinal Peptide / antagonists & inhibitors
  • Vasoactive Intestinal Peptide / metabolism
  • Vasoactive Intestinal Peptide / pharmacology*


  • Growth Substances
  • Proteins
  • Receptors, Gastrointestinal Hormone
  • Receptors, Vasoactive Intestinal Peptide
  • Secretin
  • Vasoactive Intestinal Peptide
  • DNA
  • GTP-Binding Proteins