Induction of the expression of the interleukin-1 beta gene in mouse spleen by ionizing radiation

Radiat Res. 1993 Mar;133(3):321-6.


Spleen cells freshly isolated from normal mice were irradiated with 20 Gy X rays in culture. Northern blot hybridizations revealed that expression of the interleukin-1 beta (IL-1 beta) gene was induced immediately after irradiation and was increased for 2 h thereafter. Dibutyryl cyclic AMP also caused a persistent expression of the IL-1 beta gene, although it differed from X rays in that it coincidentally induced expression of the c-fos gene, which was not induced by X rays. Activation of either protein kinase C or calmodulin also induced early expression of both IL-1 beta and c-fos. Myeloid cells collected from the spleen of mice with granulocytic leukemia were X-irradiated in culture as above. The leukemia cells responded to X rays as well as to other stimuli in the same manner as the spleen cells, except that IL-1 beta mRNA was no longer detected 30 min after irradiation while c-fos was detectable for 2 h. When the leukemia cells were irradiated twice with a 3-h interval between irradiations, the second irradiation led to prolonged expression of IL-1 beta without inducing c-fos expression. These results suggest that ionizing radiation elicits early expression of the IL-1 beta gene through a mechanism that does not involve protein kinase C or A, or the transcription factor, c-fos. Whole-body irradiation of mice with 50 Gy 137Cs gamma rays also induced IL-1 beta expression in spleen but not in bone marrow or liver, although there was a delay of several hours before it was amply expressed. Furthermore, a delay as long as 24 or 72 h was observed when the radiation dose was reduced to 8.5 or 4 Gy. The results of this in vivo study suggest that the rapidity of expression of the IL-1 beta gene is dependent on the dose of radiation, and that the cells in the body cannot respond to radiation as rapidly as cells in culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bucladesine / pharmacology
  • Calmodulin / pharmacology
  • Cesium Radioisotopes
  • Gene Expression
  • Genes, fos
  • Interleukin-1 / biosynthesis
  • Interleukin-1 / genetics*
  • Leukemia, Myeloid / pathology
  • Male
  • Mice
  • Mice, Inbred C3H
  • Protein Kinase C / metabolism
  • Radiation Genetics
  • Specific Pathogen-Free Organisms
  • Spleen / cytology
  • Spleen / drug effects
  • Spleen / radiation effects*
  • Tumor Cells, Cultured / radiation effects
  • Whole-Body Irradiation


  • Calmodulin
  • Cesium Radioisotopes
  • Interleukin-1
  • Bucladesine
  • Protein Kinase C