Negative regulation of P element excision by the somatic product and terminal sequences of P in Drosophila melanogaster

Mol Gen Genet. 1993 Feb;237(1-2):145-51. doi: 10.1007/BF00282795.

Abstract

A transient in vivo P element excision assay was used to test the regulatory properties of putative repressor-encoding plasmids in Drosophila melanogaster embryos. The somatic expression of an unmodified transposase transcription unit under the control of a heat shock gene promoter (phs pi) effectively repressed P excision in a dose-dependent manner at very low concentrations relative to somatically active transposase (encoded by the hs pi delta 2-3 gene). Maximum repression required transcription of the complete transposase gene. Dose-dependent repression of P excision was also observed in the presence of a vector plasmid (pCarnegie4) having only the terminal sequences, including transposase binding sites, of the P element. However, repression required considerably higher concentrations of pCarnegie4 than phs pi, and elimination of P excision was not observed.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Blotting, Northern
  • Chromosomes
  • DNA Transposable Elements / genetics*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / enzymology
  • Drosophila melanogaster / genetics*
  • Gene Expression Regulation, Enzymologic*
  • Genetic Vectors
  • Heat-Shock Proteins / genetics
  • Nucleotidyltransferases / genetics*
  • Plasmids / genetics
  • Promoter Regions, Genetic / genetics
  • Repressor Proteins / analysis
  • Transcription, Genetic
  • Transposases

Substances

  • DNA Transposable Elements
  • Heat-Shock Proteins
  • Repressor Proteins
  • Nucleotidyltransferases
  • Transposases