Molecular characterization of receptors for human interleukin-8, GRO/melanoma growth-stimulatory activity and neutrophil activating peptide-2

Mol Immunol. 1993 Mar;30(4):359-67. doi: 10.1016/0161-5890(93)90065-j.


Interleukin-8 (IL-8), neutrophil activating peptide-2 (NAP-2), and growth regulated gene (GRO, also known as melanoma growth stimulatory activity) are members of a family of peptides which are chemotactic agents for inflammatory cells such as neutrophils. Receptors have been identified for IL-8, GRO and NAP-2 on human neutrophils and granulocytic cell lines, and it has been observed that these cytokines can cross-compete for binding to a common receptor. Using the recently characterized rabbit IL-8 receptor as a probe, two classes of cDNAs, termed type 1 and type 2, were isolated from a human neutrophil library. The type 1 receptor binds only IL-8 while the type 2 receptor binds IL-8, GRO and NAP-2 at high affinity when respective cDNAs are expressed in COS-7 cells. The two cDNAs encode proteins that have an amino acid sequence identity of 77% while the type 1 and 2 receptors have an identity of 84 and 74% with the rabbit IL-8 receptor. These receptors also show significant homology with receptors for other chemotactic agents and with potential coding regions from the human cytomegalovirus genome.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cells, Cultured
  • Chemokine CXCL1
  • Chemokines, CXC*
  • Chemotactic Factors / metabolism*
  • DNA / isolation & purification
  • Dose-Response Relationship, Drug
  • Granulocytes / metabolism
  • Growth Substances / metabolism*
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Interleukin-8 / metabolism
  • Leukemia, Myeloid
  • Mice
  • Molecular Sequence Data
  • Neoplasm Proteins / metabolism*
  • Neutrophils / metabolism
  • Peptides / metabolism*
  • Receptors, Cell Surface / chemistry*
  • Receptors, Cell Surface / metabolism
  • Receptors, Cytokine*
  • Receptors, Immunologic / chemistry*
  • Receptors, Immunologic / metabolism
  • Receptors, Interleukin-8A
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Tumor Cells, Cultured
  • beta-Thromboglobulin


  • CXCL1 protein, human
  • Chemokine CXCL1
  • Chemokines, CXC
  • Chemotactic Factors
  • Cxcl1 protein, mouse
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-8
  • Neoplasm Proteins
  • PPBP protein, human
  • Peptides
  • Receptors, Cell Surface
  • Receptors, Cytokine
  • Receptors, Immunologic
  • Receptors, Interleukin-8A
  • beta-Thromboglobulin
  • melanoma growth stimulating activity receptor
  • connective tissue-activating peptide
  • DNA