Abstract
Interleukin-8 (IL-8), neutrophil activating peptide-2 (NAP-2), and growth regulated gene (GRO, also known as melanoma growth stimulatory activity) are members of a family of peptides which are chemotactic agents for inflammatory cells such as neutrophils. Receptors have been identified for IL-8, GRO and NAP-2 on human neutrophils and granulocytic cell lines, and it has been observed that these cytokines can cross-compete for binding to a common receptor. Using the recently characterized rabbit IL-8 receptor as a probe, two classes of cDNAs, termed type 1 and type 2, were isolated from a human neutrophil library. The type 1 receptor binds only IL-8 while the type 2 receptor binds IL-8, GRO and NAP-2 at high affinity when respective cDNAs are expressed in COS-7 cells. The two cDNAs encode proteins that have an amino acid sequence identity of 77% while the type 1 and 2 receptors have an identity of 84 and 74% with the rabbit IL-8 receptor. These receptors also show significant homology with receptors for other chemotactic agents and with potential coding regions from the human cytomegalovirus genome.
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Line
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Cells, Cultured
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Chemokine CXCL1
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Chemokines, CXC*
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Chemotactic Factors / metabolism*
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DNA / isolation & purification
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Dose-Response Relationship, Drug
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Granulocytes / metabolism
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Growth Substances / metabolism*
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Humans
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Intercellular Signaling Peptides and Proteins*
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Interleukin-8 / metabolism
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Leukemia, Myeloid
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Mice
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Molecular Sequence Data
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Neoplasm Proteins / metabolism*
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Neutrophils / metabolism
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Peptides / metabolism*
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Receptors, Cell Surface / chemistry*
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Receptors, Cell Surface / metabolism
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Receptors, Cytokine*
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Receptors, Immunologic / chemistry*
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Receptors, Immunologic / metabolism
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Receptors, Interleukin-8A
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Sequence Alignment
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Sequence Homology, Amino Acid
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Tumor Cells, Cultured
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beta-Thromboglobulin
Substances
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CXCL1 protein, human
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Chemokine CXCL1
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Chemokines, CXC
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Chemotactic Factors
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Cxcl1 protein, mouse
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Growth Substances
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Intercellular Signaling Peptides and Proteins
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Interleukin-8
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Neoplasm Proteins
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PPBP protein, human
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Peptides
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Receptors, Cell Surface
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Receptors, Cytokine
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Receptors, Immunologic
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Receptors, Interleukin-8A
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beta-Thromboglobulin
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melanoma growth stimulating activity receptor
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connective tissue-activating peptide
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DNA