Human rheumatoid synovial cells (HRSC) in culture express TGF-beta receptors and are growth stimulated by the factor

Agents Actions Suppl. 1993;39:133-7. doi: 10.1007/978-3-0348-7442-7_15.

Abstract

TGF-beta (5 ng/ml) increased the proliferation rate of HRSC by 30% after 96 h of treatment. One type of TGF-beta binding system, with apparent Kd of 953 pM and a number of 29,400 receptors/cell, was detected by Scatchard analysis of [125I]-TGF-beta binding. However, crosslinking experiments and SDS-PAGE separation showed five TGF-beta binding proteins: 50, 70, 110, 140 and 400 kDa. We may suggest that the affinities of these receptors are too close to be revealed by Scatchard plot. All together, the data suggest that TGF-beta plays a role in the hyperplasia of RA synovial tissue.

MeSH terms

  • Arthritis, Rheumatoid / metabolism*
  • Autoradiography
  • Cell Division / drug effects
  • Cells, Cultured
  • Cross-Linking Reagents / pharmacology
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Iodine Radioisotopes
  • Kinetics
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Transforming Growth Factor beta
  • Stimulation, Chemical
  • Synovial Membrane / cytology
  • Synovial Membrane / metabolism*
  • Transforming Growth Factor beta / pharmacology*

Substances

  • Cross-Linking Reagents
  • Iodine Radioisotopes
  • Receptors, Cell Surface
  • Receptors, Transforming Growth Factor beta
  • Transforming Growth Factor beta