Cloning and expression of murine CD27: comparison with 4-1BB, another lymphocyte-specific member of the nerve growth factor receptor family

Eur J Immunol. 1993 Apr;23(4):943-50. doi: 10.1002/eji.1830230427.


CD27 is a member of the nerve growth factor receptor family, that includes two types of tumor necrosis factor receptor, CD40 and Fas/Apo-1. Human CD27 has been found only on lymphocytes. In T cells, its expression strongly increases in a transient fashion upon antigenic stimulation, suggesting that CD27 plays a role during T cell activation. To analyze the function of CD27, we have identified the murine CD27 at the cDNA and protein level. Murine CD27 shows an identity of 65% compared with human CD27. The amino-terminal cysteine-rich region, i.e. the putative ligand-binding domain, and the carboxy-terminal part of the cytoplasmic domain are approximately 80% identical in man and mouse. Murine CD27 has 29% identity to 4-1BB, another lymphocyte-specific member of the receptor family defined only at the cDNA level. Murine CD27 and 4-1BB have 39% homology in the cysteine-rich domain and share a conserved region in the cytoplasmic tail. Expression studies identified murine CD27 mRNA in thymus and spleen, but not in non-lymphoid tissues, while 4-1BB mRNA was not detected in any tissue tested. In resting T cells, only murine CD27 mRNA was found, while in activated T cells murine CD27 as well as 4-1BB were present at high levels. Murine CD27 and 4-1BB mRNA are expressed with different kinetics during T cell activation, suggesting that these molecules play different roles in this process. Peptide antisera identified murine CD27 as a 45-kDa protein on thymocytes and activated T cells, while 4-1BB was precipitated as a 35-40-kDa protein from activated T cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, CD / genetics*
  • Antigens, CD / metabolism
  • Antigens, Differentiation, T-Lymphocyte / genetics*
  • Antigens, Differentiation, T-Lymphocyte / metabolism
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Humans
  • Lymphocyte Activation
  • Membrane Glycoproteins / genetics
  • Mice
  • Molecular Sequence Data
  • Multigene Family
  • RNA, Messenger / genetics
  • Receptors, Cell Surface / genetics*
  • Receptors, Nerve Growth Factor / genetics
  • Receptors, Tumor Necrosis Factor*
  • Sequence Alignment
  • T-Lymphocytes / physiology*
  • Tissue Distribution
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Tumor Necrosis Factor Receptor Superfamily, Member 9


  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • Membrane Glycoproteins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Nerve Growth Factor
  • Receptors, Tumor Necrosis Factor
  • TNFRSF9 protein, human
  • Tnfrsf9 protein, mouse
  • Tumor Necrosis Factor Receptor Superfamily, Member 7
  • Tumor Necrosis Factor Receptor Superfamily, Member 9
  • DNA

Associated data

  • GENBANK/L24495
  • GENBANK/X66042
  • GENBANK/X66043
  • GENBANK/X66045
  • GENBANK/X66046
  • GENBANK/X67167
  • GENBANK/Z18316
  • GENBANK/Z18317
  • GENBANK/Z18318
  • GENBANK/Z18319