Differential effects of 15-HPETE and 15-HETE on BHK-21 cell proliferation and macromolecular composition

Biochim Biophys Acta. 1993 Mar 17;1167(1):102-8. doi: 10.1016/0005-2760(93)90223-v.


Arachidonate and/or linoleate metabolites have been implicated in modulating cell growth, replication and cell transformations. In studies with BHK-21 cells, we found lipoxygenase and cyclooxygenase inhibitors (NDGA and indomethacin, respectively) to be antiproliferative. Studies on the metabolism of arachidonic acid in BHK-21 cells have demonstrated that prostaglandin D2 is the major cyclooxygenase product, and 15-hydroxyeicosatetraenoic acid (15-HETE) is the major lipoxygenase product. Addition of D2 showed a significant decrease in the BHK-21 cell number showing antiproliferative action. Addition of lipoxygenase products, on the other hand, showed differential effects in that 15-HPETE decreased the cell number while 15-HETE increased. NDGA and 15-HPETE decreased DNA, RNA and protein contents, while 15-HETE significantly increased them. 5-HPETE and 5-HETE also showed similar results but were less potent than 15-H(P)ETEs. The differential effects of 15-HPETE and 15-HETE could be due to the generation of free radicals by the hydroperoxide and mitogenic response by hydroxide.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arachidonate 5-Lipoxygenase / metabolism
  • Cell Division / drug effects
  • Cell Line / drug effects
  • Cricetinae
  • Cyclooxygenase Inhibitors / pharmacology
  • Hydroxyeicosatetraenoic Acids / pharmacology*
  • Kidney / drug effects*
  • Leukotrienes / pharmacology*
  • Lipid Peroxidation / drug effects
  • Lipid Peroxides / pharmacology*
  • Lipoxygenase Inhibitors
  • Macromolecular Substances
  • Peroxidase / metabolism


  • Cyclooxygenase Inhibitors
  • Hydroxyeicosatetraenoic Acids
  • Leukotrienes
  • Lipid Peroxides
  • Lipoxygenase Inhibitors
  • Macromolecular Substances
  • 15-hydroperoxy-5,8,11,13-eicosatetraenoic acid
  • 15-hydroxy-5,8,11,13-eicosatetraenoic acid
  • Peroxidase
  • Arachidonate 5-Lipoxygenase