Prenatal diagnosis of cytomegalovirus (CMV) infection: a preliminary report

Prenat Diagn. 1993 Feb;13(2):131-6. doi: 10.1002/pd.1970130208.


Eight patients were referred for prenatal diagnosis for suspected fetal cytomegalovirus infection (CMV): six for documented first-trimester infection and two for abnormal ultrasound evaluation suggestive of fetal infection. Three methods of diagnosis were employed: (1) amniotic fluid viral cultures and CMV-specific IgM in fetal serum; (2) amniotic fluid cultures and detection by polymerase chain reaction amplification of CMV-specific DNA in chorionic villi; and (3) detection of CMV-specific DNA in villus samples only. Amniotic fluid cultures detected all cases of infection, but CMV-specific IgM was not a reliable indicator of infection in any case. DNA analysis correlated well with both culture results and clinical outcome.

MeSH terms

  • Amniocentesis
  • Amniotic Fluid / microbiology
  • Antibodies, Viral / blood
  • Chorionic Villi Sampling
  • Cordocentesis
  • Cytomegalovirus / genetics
  • Cytomegalovirus / immunology
  • Cytomegalovirus / isolation & purification
  • Cytomegalovirus Infections / diagnosis*
  • DNA, Viral / analysis
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fetal Blood / immunology
  • Fetal Diseases / diagnosis*
  • Humans
  • Immunoblotting
  • Immunoglobulin M / blood
  • Polymerase Chain Reaction
  • Pregnancy
  • Pregnancy Complications, Infectious
  • Pregnancy Trimester, First
  • Pregnancy Trimester, Second
  • Prenatal Diagnosis / methods*


  • Antibodies, Viral
  • DNA, Viral
  • Immunoglobulin M