Primary structure and functional expression of the omega-conotoxin-sensitive N-type calcium channel from rabbit brain

Neuron. 1993 Apr;10(4):585-98. doi: 10.1016/0896-6273(93)90162-k.


The complete amino acid sequence of a rabbit brain calcium channel (BIII) has been deduced by cloning and sequencing the cDNA. The open reading frame encodes 2339 amino acids, which corresponds to an M(r) of 261,167. A phylogenetic tree representing evolutionary relationships indicates that BIII is grouped together with the other rabbit brain calcium channels, BI and BII, into a subfamily that is distinct from the dihydropyridine-sensitive L-type subfamily. Transient expression in cultured skeletal muscle myotubes derived from muscular dysgenic mice demonstrates that the BIII channel mediates an omega-conotoxin-sensitive calcium current with kinetics and voltage dependence like those previously reported for whole-cell N-type current. Cell-attached patch recordings, with isotonic barium as the charge carrier, revealed distinct single channels with an average slope conductance of 14.3 pS.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Brain / metabolism*
  • Calcium Channel Blockers / pharmacology
  • Calcium Channels / drug effects
  • Calcium Channels / genetics
  • Calcium Channels / metabolism*
  • Histocytochemistry
  • In Situ Hybridization
  • Molecular Sequence Data
  • Peptides, Cyclic / pharmacology*
  • Rabbits
  • Tissue Distribution
  • omega-Conotoxins*


  • Calcium Channel Blockers
  • Calcium Channels
  • Peptides, Cyclic
  • omega-Conotoxins
  • Conus magus toxin

Associated data

  • GENBANK/D14157