Cloning of a novel putative G-protein-coupled receptor (NLR) which is expressed in neuronal and lymphatic tissue

FEBS Lett. 1993 Apr 26;321(2-3):173-8. doi: 10.1016/0014-5793(93)80102-z.

Abstract

A novel G-protein-coupled receptor was isolated from mouse and rat neuronal and lymphatic tissues. The amino acid sequence of the rat receptor (rNLR) shows an overall homology of 80% to a recently cloned receptor from Burkitt's lymphoma cells (BLR1) which is exclusively expressed in lymphatic tissues [(1992) Eur. J. Immunol. 22, 2795]. Much less homology between rNLR and BLR1 was observed at the N-terminus (about 40%), whereas rNLR and the mouse homologue mNLR show 92% amino acid identity. Northern blot analysis of NLR revealed a predominant 5.5 kb mRNA species in various brain regions and neuronal cell lines, whereas in the spleen a 3 kb transcript is predominant. This distribution suggests a role of NLR in the nervous and immune systems.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism
  • Burkitt Lymphoma
  • Cloning, Molecular / methods
  • GTP-Binding Proteins / genetics*
  • GTP-Binding Proteins / metabolism
  • Genomic Library
  • Humans
  • Introns
  • Lymphocytes / metabolism*
  • Mice
  • Molecular Sequence Data
  • Neurons / metabolism*
  • Oligodeoxyribonucleotides
  • Organ Specificity
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism
  • Receptors, G-Protein-Coupled*
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Tumor Cells, Cultured

Substances

  • CXCR5 protein, mouse
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • GTP-Binding Proteins