Identification and characterization of adenosine A1 receptor-cAMP system in human glomeruli

Kidney Int. 1993 Apr;43(4):928-32. doi: 10.1038/ki.1993.130.

Abstract

Although adenosine is known to affect renal function through stimulating adenosine receptors, little is known about A1 receptors in human glomeruli. Thus, we attempted to identify the adenosine A1 receptor-cyclic AMP (cAMP) system in human glomeruli. Normal renal cortical tissues were obtained at nephrectomy of patients with renal cell carcinoma. Glomeruli were isolated using a graded sieving method or dissected manually under a stereomicroscope. Radioligand binding assay using 2-chloro-N-[3H] cyclopentyl adenosine ([3H]CCPA, an A1 agonist ligand) was performed at 30 degrees C for 90 minutes. Cyclic AMP (cAMP) produced in glomeruli was measured after incubation with different concentrations of N6-cyclohexyladenosine (CHA; A1 agonist) and a phosphodiesterase inhibitor. The specific binding was saturated within 60 minutes and reversible by adding 1 mM of theophylline. Scatchard plot analysis revealed a single class of binding site (Kd = 1.78 +/- 0.21 nM, Bmax = 271.7 +/- 35.8 fmol/mg protein). The specific binding was inhibited dose-dependently by various agents in an order suggesting A1 receptor specificity. CHA inhibited the production of cAMP in microdissected human glomeruli. This inhibitory effect was antagonized by 8-cyclopentyl-1,3-dipropylxanthine (DPCPX; A1 antagonist). This is the first study revealing the presence of the A1 receptor-cAMP system in human glomeruli using a radioligand binding assay method and by measuring the cAMP production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine / analogs & derivatives
  • Adenosine / metabolism
  • Adenosine / pharmacology
  • Binding, Competitive
  • Cyclic AMP / biosynthesis*
  • Humans
  • In Vitro Techniques
  • Kidney Glomerulus / drug effects
  • Kidney Glomerulus / metabolism*
  • Kinetics
  • Radioligand Assay
  • Receptors, Purinergic / drug effects
  • Receptors, Purinergic / metabolism*
  • Xanthines / pharmacology

Substances

  • Receptors, Purinergic
  • Xanthines
  • N(6)-cyclohexyladenosine
  • 2-chloro-N(6)cyclopentyladenosine
  • 1,3-dipropyl-8-cyclopentylxanthine
  • Cyclic AMP
  • Adenosine