Neutrophil-activating peptide (interleukin-8) in colonic mucosa from patients with Crohn's disease

Scand J Gastroenterol. 1993 Apr;28(4):296-300. doi: 10.3109/00365529309090244.


We considered the role of two neutrophil chemotactic agents (interleukin-8 and leukotriene B4) and of myeloperoxidase (a neutrophil-associated enzyme) in the pathologic condition of Crohn's disease (CD). Serial biopsy samples were taken at different sites in the colon, washed in 0.02 M phosphate-saline buffer, homogenized, and then sonicated. Interleukin-8 levels were significantly increased throughout the colonic mucosa (> 300 pg/mg protein) in patients with CD compared with control groups (< 40 pg/mg protein) (p < or = 0.01). A two- to six-fold increase in leukotriene B4 was also found in CD, whereas mucosal levels of myeloperoxidase were unchanged compared with control subjects. This study demonstrates that interleukin-8 and leukotriene B4 may have an immunologic role in the pathologic condition of CD.

MeSH terms

  • Adult
  • Aged
  • Chromatography, High Pressure Liquid
  • Colon / chemistry*
  • Crohn Disease / metabolism*
  • Humans
  • Interleukin-8 / analysis*
  • Intestinal Mucosa / chemistry*
  • Leukotriene B4 / analysis
  • Middle Aged
  • Peroxidase / analysis
  • Radioimmunoassay


  • Interleukin-8
  • Leukotriene B4
  • Peroxidase