A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions

EMBO J. 1993 May;12(5):1997-2003.

Abstract

Transcription of the Saccharomyces cerevisiae CTT1 gene encoding the cytosolic catalase T is activated by a variety of stress conditions: it is derepressed by nitrogen starvation and induced by heat shock. Furthermore, it is activated by osmotic and oxidative stress. This study shows that a CTT1 upstream region previously found to be involved in nitrogen, cAMP and heat control (base pairs -382 to -325) contains a UAS element (STRE, -368 to -356), which is sufficient for the activation of a reporter gene by all types of stress acting on CTT1. Gel retardation experiments demonstrated the existence of a factor specifically binding to STRE, but to a lesser extent to mutated elements having partly or entirely lost the ability to mediate stress control. Heat activation of STRE, but not of a canonical heat shock element, is enhanced by a ras2 defect mutation, which enhances thermotolerance, and is dramatically reduced by a bcy1 disruption mutation, which decreases thermotolerance. It can be hypothesized, therefore, that the novel stress control element is important for the establishment of induced stress tolerance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Catalase / genetics*
  • Cyclic AMP / metabolism
  • DNA, Fungal / genetics
  • DNA, Fungal / metabolism
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Hot Temperature
  • Molecular Sequence Data
  • Nitrogen / metabolism
  • Osmosis
  • Oxidation-Reduction
  • Promoter Regions, Genetic
  • Protein Kinases / metabolism*
  • Regulatory Sequences, Nucleic Acid*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Transcription, Genetic*
  • Transcriptional Activation

Substances

  • DNA, Fungal
  • Fungal Proteins
  • Cyclic AMP
  • Catalase
  • Protein Kinases
  • Nitrogen