Abstract
To investigate whether the feline CD 4 (fCD4) molecules are involved in infections of highly lymphotropic feline immunodeficiency virus (FIV) isolates, we expressed fCD4 stably on Crandell feline kidney cells and Felis catus whole foetus 4 cells by transfection of a cDNA encoding the fCD4 glycoprotein, and then infected them with TM 1 and TM 2 strains of FIV, which are unable to infect these cells productively. In spite of fCD 4 being expressed on these cells, no virus production was observed. This result indicates that fCD 4 expression alone cannot induce a productive infection of the FIV TM 1 and TM 2 strains.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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CD4 Antigens / biosynthesis
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CD4 Antigens / genetics
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CD4 Antigens / immunology*
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Cats / immunology*
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Cell Line
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DNA, Viral / genetics
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Fetus
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Flow Cytometry
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Fluorescent Antibody Technique
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Genes, gag*
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Immunodeficiency Virus, Feline / genetics
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Immunodeficiency Virus, Feline / immunology
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Immunodeficiency Virus, Feline / physiology*
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Kidney
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Plasmids
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Polymerase Chain Reaction
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / immunology
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Transfection
Substances
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CD4 Antigens
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DNA, Viral
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Oligodeoxyribonucleotides
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Recombinant Proteins