Side-chain mobility of the beta-lactamase A state probed by electron spin resonance spectroscopy

Biochemistry. 1993 Jun 1;32(21):5644-9. doi: 10.1021/bi00072a021.


beta-Lactamase from Bacillus licheniformis forms a stable compact intermediate state at low pH and moderate salt concentration (the A state), with properties consistent with a molten globule. A single cysteine residue was introduced into this class A beta-lactamase by site-directed mutagenesis at position 166. A spin label was attached to the thiol of this cysteine residue via a disulfide bond as a probe of the side-chain mobility. The mutant protein and the spin-labeled derivative exhibited similar conformational properties to the wild-type enzyme at acidic pH. The A state induced by chloride or trichloroacetate (TCA) anions was characterized by circular dichroism and esr. The A state at pH 0.5 (0.32 M HCl), or at pH 2 in the presence of 8 mM TCA or 0.4 M Cl-, had comparable amounts of secondary structure to the native state but lacked significant tertiary structure, as judged by the lack of near-UV circular dichroism. Analysis of the esr spectral line widths showed that the mobility of the spin label in the A state was similar to that in the native state and much less mobile than in the unfolded state, indicating significant constraints on the side-chain mobility in this region of the molecule in the A state. The implications of this finding to the structure of the A state are discussed.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacillus / enzymology
  • Circular Dichroism
  • Cysteine
  • Electron Spin Resonance Spectroscopy
  • Hydrogen-Ion Concentration
  • Mutagenesis, Site-Directed
  • Protein Conformation*
  • Protein Denaturation
  • Protein Folding
  • Recombinant Proteins / chemistry
  • Spectrophotometry, Ultraviolet
  • Spin Labels
  • Thermodynamics
  • Urea
  • beta-Lactamases / chemistry*
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism


  • Recombinant Proteins
  • Spin Labels
  • Urea
  • beta-Lactamases
  • Cysteine