Identification of a DNA-binding Protein of Human Herpesvirus 6, a Putative DNA Polymerase Stimulatory Factor

J Gen Virol. 1993 Jun;74 ( Pt 6):1003-9. doi: 10.1099/0022-1317-74-6-1003.

Abstract

A 41K early nuclear antigen (p41), expressed in human herpesvirus type 6 (HHV-6)-infected T cells, was cloned by screening a cDNA expression library with the anti-p41 monoclonal antibody (MAb) C5. When expressed in mammalian cells, the cloned p41 protein comigrated with the authentic p41 protein from HHV-6-infected cells and localized to the nucleus. HHV-6 p41 shares 44% sequence identity with the human cytomegalovirus (HCMV) DNA-binding protein, ICP36 (UL44 gene product); p41 binds to ssDNA with the same apparent affinity as ICP36. Since ICP36 has recently been shown to be an HCMV DNA polymerase-associated stimulatory factor, a similar function is suggested for p41.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA, Single-Stranded / metabolism
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / pharmacology
  • DNA-Directed DNA Polymerase / drug effects
  • Herpesvirus 6, Human / genetics*
  • Humans
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Sequence Homology, Amino Acid
  • Viral Proteins / genetics*
  • Viral Proteins / metabolism
  • Viral Proteins / pharmacology

Substances

  • DNA, Single-Stranded
  • DNA-Binding Proteins
  • ICP36 protein, Cytomegalovirus
  • RNA, Messenger
  • Viral Proteins
  • p41 protein, Human herpesvirus 6
  • DNA-Directed DNA Polymerase

Associated data

  • GENBANK/L12003