Characterization of the UL10 Gene Product of Herpes Simplex Virus Type 1 and Investigation of Its Role in Vivo

J Gen Virol. 1993 Jun;74 ( Pt 6):975-83. doi: 10.1099/0022-1317-74-6-975.

Abstract

On the basis of predicted amino acid sequence characteristics, herpes simplex virus type 1 gene UL10 is thought likely to encode a membrane protein with eight potential transmembrane regions. Previously, a protein with an apparent M(r) 47,000 on SDS-PAGE was identified as a product of this gene. Here we have further characterized this protein, and show that it is modified by N-linked glycosylation, associates with membranes from infected cells, and is a component of the virus particle. It is not essential for virus growth in tissue culture. To investigate its role in vivo a deletion mutant lacking the majority of the UL10 open reading frame was constructed (UL10-del). The in vitro growth properties of this virus were consistent with previous studies; it grew to give slightly lower yields than wild-type and revertant viruses, and had no apparent temperature-sensitive or host range phenotype. In vivo, in a mouse model, UL10-del was capable of establishing a latent infection, although it was impaired for growth at the periphery, and for spread to and/or growth within the nervous system relative to wild-type or revertant viruses.

MeSH terms

  • Animals
  • Female
  • Gene Deletion
  • Genes, Viral / genetics
  • Glycoproteins / chemistry
  • Glycosylation
  • Membrane Glycoproteins*
  • Membrane Proteins / chemistry*
  • Membrane Proteins / genetics
  • Membranes / chemistry
  • Mice
  • Mice, Inbred BALB C
  • Simplexvirus / chemistry*
  • Simplexvirus / genetics
  • Simplexvirus / pathogenicity
  • Viral Proteins / chemistry*
  • Viral Proteins / genetics
  • Virion / chemistry
  • Virion / growth & development
  • Virulence
  • Virus Activation

Substances

  • Glycoproteins
  • Membrane Glycoproteins
  • Membrane Proteins
  • UL10 protein, Human herpesvirus 1
  • Viral Proteins