Structural analysis on the sugar chains of human alpha 1-antitrypsin: presence of fucosylated biantennary glycan in hepatocellular carcinoma

Arch Biochem Biophys. 1993 Jun;303(2):281-7. doi: 10.1006/abbi.1993.1284.

Abstract

Chemical structures of the sugar chains of alpha 1-antitrypsin (AAT) from patients with hepatocellular carcinoma (HCC) and from healthy individuals with a different affinity for Lens culinaris agglutinin (LCA) were examined by pyridylamination of their oligosaccharides and stepwise exoglycosidase digestion in combination with reversed-phase and size-fractionation high-performance liquid chromatography. We found that the LCA-reactive species of AAT from patients with HCC carried both the biantennary sugar chain with a fucose residue at the innermost N-acetylglucosamine residue, Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4(Fuc alpha 1-6)GlcNAc-PA, and the biantennary chain without a fucose residue, Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4GlcNAc-PA, at a ratio of about 1:0.6. The LCA-nonreactive species of AAT contained the biantennary sugar chain Gal beta 1-4GlcNAc beta 1-2Man alpha 1-6(Gal beta 1-4GlcNAc beta 1-2Man alpha 1-3)Man beta 1-4GlcNAc beta 1-4GlcNAc as a major component. These results indicate that a characteristic feature of the carbohydrate chains of AAT from patients with HCC is an increment in fucosylation.

MeSH terms

  • Carbohydrate Metabolism
  • Carbohydrate Sequence
  • Carbohydrates / chemistry*
  • Carcinoma, Hepatocellular / chemistry*
  • Chromatography, High Pressure Liquid
  • Fucose / metabolism*
  • Humans
  • Lectins / metabolism
  • Liver Neoplasms / chemistry*
  • Molecular Sequence Data
  • Plant Lectins*
  • Polysaccharides / analysis*
  • alpha 1-Antitrypsin / chemistry*
  • alpha 1-Antitrypsin / metabolism
  • alpha-L-Fucosidase / metabolism

Substances

  • Carbohydrates
  • Lectins
  • Plant Lectins
  • Polysaccharides
  • alpha 1-Antitrypsin
  • lentil lectin
  • Fucose
  • alpha-L-Fucosidase